Journal: European Journal of Biochemistry

Article Title: Analysis of the thyrotropin‐releasing hormone‐degrading ectoenzyme by site‐directed mutagenesis of cysteine residues

doi: 10.1046/j.1432-1327.2000.01277.x

Figure Lengend Snippet: Subcellular distribution of the truncated GFP‐fusion proteins with either cysteine or serine at position 68, as analyzed by confocal laser fluorescence microscopy. BHK cells were transfected either with the empty pEGFP‐N1 vector (A), the EGFP‐tagged, truncated wild‐type cDNA of TRH‐DE (B) or the corresponding C68S mutant (C) and processed as described in .

Article Snippet: We thank André Wolfstein and Beate Sodeik (Institut für physiologische Chemie, Medizinische Hochschule, Hannover) for experienced support with confocal fluorescence microscopy, Lutz Schomburg for advice and helpful discussions, Uwe Grunenberg for excellent technical assistance, Valerie Ashe for linguistic help and the Deutsche Forschungsgemeinschaft for financial support.

Techniques: Fluorescence, Microscopy, Transfection, Plasmid Preparation, Mutagenesis

Journal: PLoS ONE

Article Title: TBCK Influences Cell Proliferation, Cell Size and mTOR Signaling Pathway

doi: 10.1371/journal.pone.0071349

Figure Lengend Snippet: HEK293 cells infected with the lentiviruses targeting to the various regions of TBCK mRNA (TBCK-RNAi-1 or 2) were subjected to Western blotting ( A ), cell counts ( B ), fluorescence microscopy ( C ) and MTT analyses ( D ). Bar, 200 µm. Lentivirus-infected cells were GFP-positive. Data are shown as the mean of three independent experiments ± SE (* P <0.05, ** P <0.01).

Article Snippet: The mounted coverslips were analyzed with confocal fluorescence microscopy (LSM510 Meta; Carl Zeiss).

Techniques: Infection, Western Blot, Fluorescence, Microscopy

Journal: PLoS ONE

Article Title: TBCK Influences Cell Proliferation, Cell Size and mTOR Signaling Pathway

doi: 10.1371/journal.pone.0071349

Figure Lengend Snippet: HEK293 cells were infected with the indicated lentivirus and subjected to fluorescence microscopy (A–B) and FACS analysis (C–D). ( A – B ) GFP-positive signals indicate the cells infected by lentivirus. Bar, 20 µm. The area measurements of infected cells were quantified by Image-Pro Plus 6.0 software. Data are shown as the mean of three independent experiments ± SE (** P <0.01, n>200). ( C – D ) Representative histogram of flow cytometry shows the size distribution (FSC-H) of GFP positive cells that were stained with propidium iodide. The mean of three independent experiments ± SE is shown (* P <0.05, n>100,000).

Article Snippet: The mounted coverslips were analyzed with confocal fluorescence microscopy (LSM510 Meta; Carl Zeiss).

Techniques: Infection, Fluorescence, Microscopy, Software, Flow Cytometry, Staining